Purification and characterization of testicular transferrin secreted by rat Sertoli cells
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چکیده
منابع مشابه
Purification and characterization of testicular transferrin secreted by rat Sertoli cells.
Sertoli cells synthesize and secrete a transferrin-like protein (testicular transferrin) [Skinner & Griswold (1980) J. Biol. Chem. 255, 1923-1925]. The purpose of the present study was to purify and characterize testicular transferrin and to compare it with serum transferrin. Testicular transferrin was obtained from the medium of cultured rat Sertoli cells, whereas serum transferrin was obtaine...
متن کاملSecretion of testicular transferrin by cultured Sertoli cells is regulated by hormones and retinoids.
We have previously reported that Sertoli cells in culture secrete a transferrin-like protein (Skinncr and Griswold, 1980). The purpose of this study was to determine to what extent hormones such as follicle-stimulating hormone (FSH), insulin and testosterone, and vitamin A can regulate the amount of testicular transferrin secreted by cultured Sertoli cells. Sertoli cell culture medium was colle...
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Growth-associated alterations in the oligosaccharide structures of transferrin secreted by HepG2 cells were examined by concanavalin A-crossed affinoimmuno-electrophoresis. Slowly dividing, confluent cultures produced transferrin with a approximately 4.5-fold greater proportion of biantennary complex-type oligosaccharides than rapidly dividing, subconfluent cultures. The activity of N-acetylglu...
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A new method is presented for the purification of testicular hyaluronidase involving ion exchange chromatography followed by gel filtration on Sephadex G-75. A highly purified hyaluronidase preparation has been obtained which contains 45,000 National Formulary activity units per mg, dry weight, and which migrates as a single component on polyacrylamide gel electrophoresis at pH 4.3. The enzyme ...
متن کاملSertoli cells synthesize and secrete transferrin-like protein.
One of the major proteins secreted by Sertoli cells (Band 3) has a molecular weight on sodium dodecyl sulfate-polyacrylamide gels which corresponds to rat serum transferrin. The preparation of Sertoli cell-secreted proteins contains an iron-binding protein with electrophoretic properties on native polyacrylamide gels similar to serum transferrin. Antibodies against Sertoli cell-secreted protein...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1984
ISSN: 0264-6021,1470-8728
DOI: 10.1042/bj2180313